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Greater Capital Region
DNA
Science Project Survey Results

BACKGROUND

The Greater Capital Region Teacher Center offers professional development for teachers, by teachers.  Funded by the NYS Legislature, teacher centers are governed by a policy board that has a majority of teachers. GCRTC serves 93 school districts, four BOCES and 68 private schools or approximately 22,000 educators.

In the summer of 1987 a few biology teachers, led by Linda H. Austin, a high school teacher from Greenwich CSD in rural upstate NY, came to the Center with a dream of offering high powered professional development to biology and life science teachers in our region.  Fresh from an intensive training in advanced DNA protocols from the DNA Learning Center at Cold Spring Harbor, these teachers were searching for a way to both extend the training that they received and to acquire the expensive equipment necessary to reproduce the labs for students. The Center supported the design and delivery of the summer institutes during the early years. In 1993 Center joined the State University at Albany and Daniel L. Wulff.  Together they wrote a successful Hughes Foundation Grant that included a teacher-training component. Within five years the Hughes Foundation Grant ended but the Teacher Center support continued. The Teacher Center bought the first DNA lab kit for about $15,000.  The lab kit was used to teach the summer institute and was available for loan to teachers trained in the protocols.  As of August 2002, one hundred and twenty five teachers have been trained in advanced DNA protocols and seven equipment kits are in circulation in the Greater Capital Region.  As a result of this project thousands of students have participated in hands on labs at the cutting edge of DNA research.

PURPOSE

In the spring of 2002 the Greater Capital Region Teacher Center decided to do a program evaluation of the Greater Capital Region DNA Science Project strand.  The purpose of this program evaluation was to ascertain the level of impact that these programs have had on teachers and students in our region and to discover what new challenges face biology and life science teachers as they work to bring the most relevant and current research into their classrooms. Initially these labs were offered to only the brightest and most motivated high school students. Beginning in 2002, all students were tested on the subject of DNA science on statewide Regents exams that all students must pass in order to graduate.  The inclusion of all students in life science and biology courses has introduced new opportunities and challenges for teachers.  Our evaluation also focused on finding out if our current support for teachers was still meeting their needs and how we might refine that support to help them meet new state and national mandates for student learning.

METHODS

A survey was sent to the 125 life science and biology teachers who completed coursework on DNA protocols.  Fifty-two teachers (41%) completed and returned the survey before June 30, 2002.

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REFLECTIONS /CONCLUSIONS/NEXT STEPS

While the majority of participants in the DNA Science Project since 1987 have been senior high school science teachers and about 14% junior high teachers, new state requirements that middle school students demonstrate in-depth knowledge of DNA will require more middle school teachers be given this training.  It should be noted that our strongest response came from those teachers who participated in the program when there was substantial grant funded financial support.  Such support would undoubtedly deepen and widen our effectiveness.

As a science teacher, this experience has enabled me to read current journal articles on Molecular Biology and stay current with my science student’s research.  It has also helped me to guide and introduce all of my students to DNA research.

Nearly 90% of the participants felt that their participation in the DNA Science Project had a significant impact on their professional growth.

Because of taking this course, I teach a lot more about genetics, recombinant technology and molecular biology.  It allowed me to understand the concepts so that I could teach them to my students.  Also taking this course has allowed me to help one of my science research students whose topic was genetically modified crops.

Over eighty percent of the participants felt that the DNA Science Project increased their confidence to teach about molecular biology labs.

Nearly 80% report they have integrated the knowledge learned from the DNA Science Workshop into their curriculum, resulting in a high degree of enhancement in their teaching, in contrast to the more typical situation where teachers receive excellent professional development opportunities with great enthusiasm, but find that translating that new knowledge into the classroom presents insurmountable challenges. The active barrowing of the seven DNA equipment kits is testimony to the fact that teachers are implementing what they have learned in the classroom.

Being able to borrow the equipment and share my “expertise” with students has been the most rewarding part of my 26 years in teaching.  It is a truly exciting experience when students have the opportunity to participate in these labs.  They remember it for years afterwards! That “Eureka” moment when they see their first gel is so great!

Seventy-three percent of the participants felt that the DNA Science Project had provided more hands-on, problem-solving exercises for students, increasing their interest and motivation.

I have always found genetic engineering and DNA profiling and DNA profiling to be fascinating and exciting.  It was probably because I was able to learn so much about these topics using the GCRTC equipment that I have been motivated to participate in the Cornell Institute for Biology Teachers this fall.

More than half of the participants felt that the DNA Science Project allowed for a greater opportunity to network with other teachers and to continue taking other courses and workshops at the Teacher Center.

Nearly half of the participants reported that the Science Project decreased their sense of isolation and re-ignited their interest in teaching.

I had one student graduating this year who decided to go into molecular biology as a career- she didn’t even know what that was in September.  

Two students who first experiences biotechnology in my classroom now have their PhD’s in Molecular Biology and felt this course opened their eyes to that field.  In addition, a recent graduate of Dartmouth College and Rhodes Scholar, Megan Steven, was inspired to enter biology after doing the DNA course

Significantly, almost half of the participants report that students have been inspired to pursue a career in biotechnology/molecular biology because of the DNA science labs.

Less than 20% felt they could attribute the DNA Science labs to improved student performance on tests. Perhaps state exams are not as accurate a measure of what students know and can do, as we would like to believe.

There were some barriers to introducing advance biotechnology into high school classrooms.

Some teachers found that there isn’t enough time to teach these complex, hands-on labs.

Nearly 10% of those who responded noted the difficulty of introducing expensive lab equipment to large classes that included all students.

Some teachers noted the difficulty when purchasing the chemicals needed to complete labs

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Teachers recommended that the Teacher Center:

  • Purchase supplies in bulk and pass the savings on to schools

  • Train new teachers (many have retired)

  • Offer further assistance in implementation of labs

 

RESULTS

Demographics

Respondents were asked a number of demographic questions to determine their current professional status, when they attended the workshop, and what grade level they typically taught or currently teach.

Seventy-six percent of those who responded took the workshop during those years that the Hughes Foundation provided substantial additional support.  The summer institutes offered from 1993 to 1997 with Hughes Foundation support provided a stipend, graduate credit, technical support and materials and supplies.

The majority of participants in the DNA Science Project were senior high science teachers.  The 12% of participants who were science department chairs were also senior high science teachers.  A small, but substantial amount of participants in the Greater Capital Region DNA Science Project were junior high science teachers (14% or 7 of the 52 returned surveys).

Eighty percent of the participants generally taught sophomore year science classes, less than half of the participants taught freshman level and junior and senior level science classes.  Many participants taught more than one grade level; those who taught more than one grade level also all taught sophomore year sciences.  Less than 20% of the participants taught junior level science classes, and none of the participants taught science classes at a level above high school.

Eighty percent of the participants taught Living Environment/Biology.  This data coincides with the data stating 80% of the participants taught sophomore level sciences, because Living Environment/ Biology is a sophomore level science.  Physical Science is a science course often taught in junior level school and the percentage relation to the amount of participants who teach junior level and the amount of participants teaching Physical Science is roughly the same.  Adding together the percentages of AP Biology and other advanced courses, roughly 47% of the participants teach advanced level courses to students.  (See Appendix A for complete results)

Table 1: Professional Status of Respondents

Profession

Junior High
Science Teachers

Senior High
Science Teachers

Science
Dept. Chair

Retired (yr?)

OTHER

# of people

                      7

                    37

               6

        8

    2

% of people

                    14%

                   73%

           12%

     16%

4%

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Table 2: Grades Taught

Grade level

7th

8th

9th

10th

11th

12th

Junior College

OTHER

# of people

8

5

23

41

16

21

0

0

% of people

16%

10%

45%

80%

31%

41%

0%

0%

Table 3: Subjects Taught

Subjects

Physical Science

Life Science

Earth Science

Living Environment/Biology

Chemistry

Physics

AP Bio

Other

# of people

8

11

8

41

8

2

9

15

% of people

16%

22%

16%

80%

16%

4%

18%

29%

 

Use of GCRTC Lab Equipment

The majority of respondents, over 75% of the returned surveys, have borrowed equipment from the Greater Capital Region DNA Science Project. 

Twelve percent noted that they do not do hands-on molecular biology labs with their students. 10% of the participants belong to school districts, which have their own equipment.

Ten percent of the respondents reported that they do not do hands-on molecular biology labs because the size of the class and the students’ range of responsibility levels are too large, making it difficult to do these types of labs with such expensive equipment.  Over five percent of the participants feel as if they do not have enough time to prepare for molecular biology labs.  Over seven percent of participants also gave free answers as reasons for not doing hands on molecular biology labs, and the themes of the answers were often related to lack of time and lack of ability to manage larger lab classes.

The most common procedures that participants in the DNA Science Project integrated into their labs were exercises to learn to use the digital micropipette and electrophoresis of dye samples.  The academic level of students who participated in these experiments ranged from advanced sciences and science research to seventh grade junior level science clubs.  Less than half of the participants performed labs that require the remaining procedures listed in the survey.  On average, students in the sophomore grade level and in honors or advanced level biology performed most of these procedures.  Many of the DNA experiments were also performed by junior level students, including electrophoresis of student-digested DNA samples, electrophoresis of pre-digested DNA samples, and Agarose gel electrophoresis of human hemoglobin samples.  Few participants integrated Polyacrylamide gel electrophoresis of other protein samples, Bacterial transformation to some other trait, and PCR procedures into their curriculum.  (See Appendix B for complete results)

Table 4: Equipment Use

 

YES

NO

# of people

39

13

% of people

76%

25%

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Table 5: Reasons for failure to use equipment

Reasons

# of people

% of people

My school has its own equipment

5

10%

I borrow equipment from CIBT

1

2%

I take my students to the DNA Learning Lab at ACC

0

0%

The hands-on molecular labs done do not require specialized equipment

3

6%

My students do not do hands-on molecular biology labs

6

12%

There isn’t enough money in my budget to purchase the necessary materials and supplies to do hands-on molecular biology labs

2

4%

I don’t have enough time to do the preparation for hands-on molecular biology labs

3

6%

There isn’t enough time in the curriculum to do hands-on molecular Biology labs

2

4%

There isn’t enough emphasis in the Core to justify the time and expense it takes to do hands-on molecular biology labs with expensive equipment

2

4%

Having all students in class makes it difficult to do hands-on molecular biology labs with expensive equipment

5

10 %

My students are too young to do hands-on molecular biology labs

1

2%

I do not teach biology or life science

4

8%

OTHER

4

8%

   

III. Impact on Teacher Professional Development

 

Nearly 90% of the participants felt that their participation in the DNA Science Project had a significant impact on their professional growth.

Eighty-two percent of the participants felt that the DNA Science Project increased their confidence to teach about molecular biology labs.  Nearly half of the participants answered that the Science Project decreased their sense of isolation and re-ignited the participant’s interest in teaching.  More than half of the participants felt that the DNA Science Project allowed for a greater opportunity to network with other teachers and to continue taking other courses and workshops at the Teacher Center.  (See Appendix C for complete responses)

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Table 7: Professional growth

 

Level

1

2

3

4

5

# of people

0

1

6

13

32

% of people

0%

2%

12%

25%

63%

 

Table 8: Impact on Teaching Career

Reason

# of people

% of people

Lessened the sense of isolation

23

45%

Increased the opportunities for networking with other teachers

32

63%

Re-ignited my interest in teaching

20

39%

Increased my confidence to teach about molecular biology

40

78%

Increased my confidence to try hands-on molecular biology labs

42

82%

Increased my confidence to take other courses/workshops

26

51%

Teachers were asked to share a personal experience that illustrates the impact of this project on their teaching career.    

Themes

 

1) The DNA Science Project has given teachers more confidence to incorporate Biotechnology more in depth into their curriculum. (12 responses in this category)

2) These classes that could not operate without the benefits of the GCRTC help explain more about what is going on in the world today (Television, Movies, etc.)   (7 responses in this category)

3)  Students conveyed most interest and viewed Biotechnology related labs and lectures as most memorable.  (6 responses in this category)

4)  Many schools could not engage in Biotechnology or related topics without the equipment provided from the DNA Science Project and the GCRTC. (6 responses in this category)

5) Teachers since taken these classes have integrated more of Genetics and Biotechnology into their curriculum with a higher confidence of the children’s ability to comprehend and enjoy the material.  (6 responses in this category)

6)  The DNA Science Project served as a refresher or a new experience for many teachers, as to which they greatly benefited.  (5 responses in this category)

7) Having Biotechnology in a student’s curriculum has motivated many students to major in a Biology/Genetic field and to pursue careers related to these fields. (5 responses in this category)

Implementation of New Knowledge into the Classroom

While teachers come away from an excellent professional development opportunity with great enthusiasm, many find that translating that new knowledge into the classroom presents insurmountable challenges. In this group however nearly 80% report they have integrated the knowledge learned from the DNA Science Workshop into their curriculum.

Seventy-six percent of the participants reported a high degree of enhancement.

Table 9: Implementation

Level

1

2

3

4

5

# of people

1

0

10

14

26

% of people

2%

0%

20%

27%

51%

Table 10: Use of DNA Labs

Level

1

2

3

4

5

# of people

0

3

8

14

25

% of people

0%

6%

16%

27%

49%

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Student Impact  

Nearly 70% of the participants stated that the workshop increased student’s interest and motivation.  72.5% of the participants felt that the DNA Science Project had provided more minds-on, problem-solving exercises for students.  Almost half of the participants report that students have been inspired to pursue a career in biotechnology/molecular biology because of the DNA science labs.  Less than 20% felt they could attribute the DNA Science labs to improved student performance on tests. 

Table 11:Student Impact

Positive impacts

# of people

% of people

Increased student’s interest and motivation

40

69%

Provided more minds-on, problem-solving exercises for students

37

73%

Improved student performance on tests

10

20%

Inspired some students to pursue a career in biotechnology/molecular biology

23

45%

OTHER

4

8%

Teachers were asked to provide a brief description of a the impact this project has had on student learning. (See Appendix D for complete responses)

 

Themes

1)   Students often remember and enjoy hands-on labs, which encourages teachers to integrate this type of course work more commonly into the curriculum.  (11 responses in this category)

2)   These Biotechnology sections in lecture and lab procedures on Biotechnology open interests in many students to enter and explore Biotechnology related fields. (10 responses in this category)

3)   Often students attain knowledge better and easier when they can apply what they have been taught in lecture to an experiment and receive results that support their lessons; the connection often sparks interest in the student to further research the subject.   (5 responses in this category)

4)  Students have shown a greater understanding of the genetics unit and biotechnology in general due to the opportunity brought to them by the DNA Science Project. (5 responses in this category).

5)  Borrowed equipment which enabled students perform labs early on in their education helped the students gain and edge and complete upper level course work with ease.  (3 responses in this category)

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How GCRTC and the Greater Capital Region DNA Project Improve Services to Life Science Teachers

Teachers were asked how GCRTC might improve services to life science and biology teachers.(See Appendix E for complete responses.)

Themes

 

1)  If Teacher Center had a variety of supplies more readily available. (6 responses in this category)

2)  Providing sessions for new teachers on how to use the materials. (4 responses in this category)

3)  Information sheets, lists, directions, and other means to help teachers get started with organizing the equipment and labs. (4 responses in this category)

4)  If the GCRTC sold supplies to the schools.  (3 responses in this category).

5)  If mini-workshops were available to teach teachers how to arrange and setup/perform labs.  (3 responses in this category)

6)  If the GCRTC could arrange mentors to assist in demonstrating procedures and techniques and to add further insight for teachers when using and performing labs. (3 responses in this category

7)  Having a location where teachers could send students to use this equipment if equipment is not directly available to them.  (2 response in this category)

8)  A method of contact for teachers to get answers from “on-hand” experts to questions regarding labs and materials.  (1 response in this category)

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SUMMARY

Based on the responses of the participants it appears that the workshops and supporting lab equipment have resulted in a number of positive impacts on what teachers and students know and can do. Positive impacts include but are not limited to:

  • Improvement in teacher knowledge

  • A high level of integration of new knowledge into the classroom

  • Improved teacher confidence in mastery of subject knowledge

  • Increased student interest in science

  • Increased students’ problem solving skills

  • Greater numbers of students entering the field of science

  • Better preparation for college level course work in science

  • Decrease in teacher isolation

There were some barriers to introducing advance biotechnology into high school classrooms.

  • Some teachers found that there isn’t enough time to teach these complex, hands-on labs

  • Nearly 10% of those who responded noted the difficulty of introducing expensive lab equipment to large classes that included all students.

Some teachers noted the difficulty when purchasing the chemicals needed to complete labs

Teachers recommended that the Teacher Center

  • Purchase supplies in bulk and pass the savings on to schools
  • Train new teachers (many have retired)
  • Offer further assistance in implementation of labs

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Appendix A Responses to Question 1

 

Class

1987 Recombinant DNA

1989 Recombinant DNA

1990 Recombinant DNA

1991 Recombinant DNA

1993 Molecular Biology

# taking class

4

6

2

3

6

% who took class

7.8%

11.7%

3.9%

5.8%

11.7%

 

 

 

 

 

 

Class

1994 Molecular Biology

1995 Molecular Biology

1996 Molecular Biology

1997 Molecular Biology

2001 Molecular Biology

# taking class

9

6

11

7

7

% who took class

17.6%

11.7%

21.5%

13.7%

13.7%

 

 

 

 

 

 

Class

OTHER

 

 

 

 

# taking  class

10

 

 

 

 

% who took class

19.6%

 

 

 

 

 

Results

(List for OTHER):

  • Woodrow Wilson Genetics Institute 1993 (2 attended)

  • Hughes Foundation Grant to Bob Parson’s at RPI – 1990 (4 weeks)  

  • Summer PCRupulate SUNYA  

  • CIBT Molecular Biology institute 1999  

  • Sum Rhine Genetics – Aug? Union College – One week

  • Biology for the New Millennium 2000 Genetics

  • Biology for the New Millennium 2001 Health & Disease

  • CSH – 1990 @ CSH – 1 wk. course

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Appendix B Responses to Question 8

 

Labs

# of people

% of people

Students/Grades/Courses

Exercise to learn to use the digital micropipette

39

76.4%

10/12/elective 12/12/Science research 8/10/honors
16/12/advanced bio  
100/10  
11/AP Bio
20-AP, 50-living environment
45/9 & 10/ Bio,11/chemistry 20/10/living environment 20/12/AP, 85/9/learning environment
15/7/science club
50/Advanced Biology
9,10,12/Liv. Envi. & AP.
50/7/Accelerated biology 23/12/AP Bio
7th & High School
Living Environment 32/10/Living Environment 80/10/Living environment 70-80/10/Living environment 50/Living environment
30/AP Bio – 50/honors Bio 65/10/General Biology 80/10/General Biology 20/Living Environment Honors
75/10/Living environment
LE 9 & 10, AP 11 & 12 20/10/Honors & 15/12/Adv. Bio
30/Applied Biology 100/9-12/Living Env. & AP 70/10-11/Living Environment 15/9 & 10/Bio/40/Integrated 80/Living Environment

Electrophoresis of dye samples

37

72.5%

12/12/Science research 8/10/honors
16/12/advanced bio
100/10 11/AP Bio  
20-AP, 50-living environment 30/ 9 & 10 living environment 20/10/living environment 20/12/AP, 85/9/learning
environment
15/7/science club 50/Regents Biology 9,10/Living Environment 50/7/Accelerated biology 23/12/AP Bio
Living Environment 32/10/Living Environment 80/10/Living Environment 70-80/10/Living environment 50/living environment 65/10/General Biology 80/10/General Biology 20/Living Environment Honors
75/10/Living environment
LE 9 & 10
15/12/Adv. Bio
30/Applied Biology
75/Living Environment 100/9-12/Living Env. & AP 70/10-11/Living Environment 15/9 & 10/Bio/40/Integrated 80/Living Environment

Electrophoresis of pre-digested DNA samples

 

 

 

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23

45%

10/12/elective 16/12/advanced bio
100/10 11/AP Bio  
20-AP, 50-living environment 20/10/living environment 10/Middle School Science Club
9,10/Living Environment 23/12/AP Bio
High School Living Environment  
32/10/Living Environment 45/10/Living Environment 80/10/General Biology 20/Living Environment Honors AP 11 & 12 100/9-12/Living Env. & AP 80/Living Environment

Electrophoresis of student-digested DNA samples

20

39.2%

10/12/elective 12/12/Science research 8/10/honors  
12/12  11/AP Bio 20-AP, 50-living environment 20/12/AP, 85/9/learning environment
50/Advanced Biology  
12/AP  
50/7/Accelerated biology
7th & High School Biology 30/AP Bio – 50 honors Bio 20/Living Environment Honors
20/12/Advanced bio students
AP 11 & 12  
20/10/Honors & 15/12/Adv. Bio 15/9 & 10/Bio/40/Integrated

Agarose gel electrophoresis of human

 hemoglobin samples

19

37.2%

12/12/Science research 16/12/advanced bio
12/12 (10th extra credit) 20-AP, 50-living environment 15/7/science club  
12/AP High School Biology 80/10/Living Environment 50/Living environment 65/10/General Biology 20/Living Environment Honors
75/10/Living Environment 30/Applied Biology 100/9-12/Living Env. & AP 15/9 & 10/Bio/40/Integrated 80/Living Environment

Agarose gel electrophoresis of other protein

samples

6

11.7%

12/12  
20/10/living environment 50/Regents Biology 23/12/AP Bio
80/Living Environment

Polyacrylamide gel electrophoresis of other

protein samples

2

3.9%

11/AP Bio
20/12/AP, 85/9/learning environment

Bacterial transformation to antibiotic resistance

21

41.1%

10/12/elective 12/12/Science research 8/10/honor
16/12/advanced bio
12/12 (10th extra credit) 11/AP Bio
20-AP, 50-living environment 50/Advanced Biology
12/AP  
23/12/AP Bio
High School Biology
Living Environment 32/10/Living Environment 70-80/10/Regents Biology 20/12/Advanced bio  
AP 11 & 12
20/10/Honors & 15/12/Adv. Bio  
15/9 & 10/Bio/40/Integrated

Bacterial transformation to bioluminescence

12

23.5%

11/AP Bio
20-AP, 50-living environment 20/12/AP, 85/9/learning
environment
High School Biology 32/10/Lving Environment
20/AP Biology 80/10/General Biology 20/12/Advanced bio 15/12/Adv. Bio 15/9 & 10/Bio/40/Integrated

Bacterial transformation to some other trait

1

1.96%

20-AP, 50-living environment

Restriction digestion of DNA samples

 

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21

41.1%

10/12/elective 12/12/Science research 8/10/honors
12/12  11/AP Bio
20-AP, 50-living environment 20/10/living environment 20/12/AP
85/9/learning environment
50/Advanced Biology
12/AP
9,12/Living environmeny & AP  
23/12/AP Bio  
High School Biology 20/Living Environment Honors  
20/12/Advanced bio  
 
AP 11 & 12  
20/10/Honors & 15/12/Adv. Bio  
100/9-12/Living Env. & AP

Ligation of DNA samples (“gene splicing”)

12

23.5%

10/12/elective 12/12/Science research 11/AP Bio
23/12/AP Bio
High School Biology 20/12/Advanced biology
 AP 11 & 12
15/12/Adv. Bio 100/9-12/Living Env. & AP

Extraction of genomic DNA

15

29.4%

8/10/honors
100/10
20-AP, 50-living environment 30/ 9 & 10 living environment 85/9/learning environment 9,12/Living envir. & AP 120/general biology
Living Environment 50/9/Living Environment 65/10/General Biology
LE 9 & 10  
20/10/Honors
75/Living Environment
15/9 & 10/Bio/40/Integrated

Extraction of plasmid DNA (“mini prep “)

11

21.5%

10/12/elective 12/12/Science research 12/12
11/AP Bio  
20/12/ AP  
50/Advanced Biology 23/12/AP Bio
20/12/Advanced Bio 15/12/Adv. Bio

PCR

4

7.8%

12/12/Science research
12/12 (New Paltz)
Living Environment

OTHER

5

9.8%

12/12/Science research 125/7/science club
Living Environment 50/10/General Biology

(List for OTHER):

  • Agarose Gel electrophoresis of lamda DNA after restricted digestion.  

  • View of equipment and how it is used and paper sample procedures are done to show the DNA fingerprinting simulation  

  • Paper Gene Splicing Lab  

  • Hydrophobic Interaction Chromatography – used to purify proteins made by the Green Fluorescent gene in bacteria


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Appendix C   
Responses to the question

If possible, please share with us a brief description of a personal experience you have had that illustrates the impact this project has had on your teaching career.

  • Several students who did this, went to major in biology and felt confident doing these labs or types of labs in college – this is a goal of mine.

  • I learned more about DNA technology to share with my students

  • My science research students were VERY bright and motivated and they (and I!!) loved the labs.  They were sorry when they ended.  We preceded the labs with a lot of lecture/discussion on molecular biology theory.  Quite a few of my students were motivated to pursue careers in biology of chemistry because of their experience; we also did a few of the labs (i.e. bacterial transformation) with 9th graders.  It was a reach for them but quite a few were excited about it.  Overall, I loved doing the labs and was very grateful that the equipment was available through the Teacher Center.  It made me a better teacher.

  • I feel that this material is important general knowledge for every student and I am the only person on the faculty who has been trained in using this equipment.

  • Hudson, a poor district, balled at providing microscopes! – We couldn’t have confirmed the program without access to the materials, courses, and updates the Teacher Center provided. And, so fewer of our students would have succeeded at top colleges.

  • With the discussions that arise from cloning and genetic engineering (in the news), I am able to explain the techniques that are used.

  • It kept me up to date with new procedures, although often in some classes there isn’t enough time to go into the depth necessary for the molecular labs.  It made them feel like “real” scientists in their worlds.  However, I have been unable to use the equipment the past 3 years, because of budget restraints and time needed to meet new standards.

  • Didn’t learn this topic as an undergrad of grad school, topic too new – learned through workshops and personal reading

  • My understanding of molecular biology directly impacts my students.  DNA is on the news all of the time and my students ask questions about what they feel.  A good example was the newest development in the Sam Shepard Case.  The students were very interested to learn how they acquitted him after all these years.  Students learn best when you counter science into their everyday life.  Molecular Biology is something they all should know to help them in the future (for example, if they sit on a jury and need to understand the evidence).

  • As a direct result of attending the DNA workshop, I developed a Biotechnology Course for accelerated 9th grade Bio students.  The course ran for 4 years, until the Living Environment curriculum came out and all variances were stopped.  It was an excellent experience for both instructors and students.  We had many students go on to major in genetics in college as a result!

  • I have retired (since 1996).  This lab work left me with a great last experience in teaching – students were really ignited by this experience.  Very timely – O.J. Simpson Trial – they really liked the experience.

  • I now incorporate more molecular Biology into my genetics unit.  Although I don’t use the electrophoresis equipment I do a simulation with the Twigg’s case.  It is more grade appropriate.  I am the only 7th grade teacher to start the genetic unit – I’ve passed labs and materials onto other teachers.

  • This course was by far the best course that I took as a postgraduate.  Thank you!

  • It is great to interact and share with other teachers – got new ideas and share mine – increases confidence in a different area.

  • It inspired me to develop a number of lab activities.  I knew that the experience of some of the students who had taken the DNA summer course was a major force for them to choose the field of Biotechnology.

  • I’m excited about the opportunity to attend the CIBT Summer Institute and get $1,500 worth of equipment for the lab.  This would not have been possible without the DNA project offerings.  Thanks!

  • would never have been able to attempt these projects without the class or the equipment.  The program is great and I really appreciate the GCRTC for making it possible.

  • Every year the majority of students select the labs done with the DNA kits as their most memorable time.

  • As a science teacher, this experience has enabled me to read current journal articles on Molecular Biology and stay current with my science student’s research.  It has also helped me to guide and introduce all of my students to DNA research.

  • We have developed a thorough and intense forensic project in our school.  The DNA kits are essential to this project!  The students always comment every year that they really enjoyed doing the electrophoresis labs.

  • Watching the student’s excitement when they actually extracted DNA was a truly worthwhile experience.  The course also gave me the courage to try other courses at my age (50’s).  Last summer I took an endangered species course at the Bronx Zoo for a week.

  • It’s great to be able to teach the lab on this subject.  The students appreciate my knowledge and time setting it up.

  • I met Linda Austin. She is great!  - Helpful, energetic, and knowledgeable.  She answered questions I had when I needed help with the labs and even shared her labs with me.  Kept me going when I hit a roadblock.

  • Nothing specific – just general overall confidence as a positive feeling from being able to transmit this to students.

  • I know it is minimal, but I make anecdotal references to the course and its content when I teach genetic engineering, DNA, etc.  If I could take a refresher class – even a 3-hour workshop, I think that it would benefit others and me.

  • Taking the CSH workshop in ’87 ignited my interest in introducing hands-on DNA science into the high school classroom.  Working with teachers from other schools to launch the GCR DNA science project ended my sense of isolation and began a long and productive association with the Teacher Center!

  • I have always found genetic engineering and DNA profiling to be fascinating and exciting.  It was probably because I was able to learn so much about these topics using the GCRTC equipment that I have been motivated to participate in the Cornell Institute for Biology Teachers institute this summer.

  • Several students became interested and followed careers in genetic engineering.

  • Allowed me access to materials that I wouldn’t have had.

  • Because of taking this course, I teach a lot more about genetics, recombinant technology and molecular biology.  It allowed me to understand the concepts so that I could teach them to my students.  Also taking this course has allowed me to help one of my science research students whose topic is genetically modified crops.

  • Motivated me to write, I teach Advanced Forensic Science to seniors already taught “Crime Lab” to Advanced 10th.

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  Appendix D

  Responses to the question

If possible, please share with us a brief description of a personal experience you have had that illustrates the impact this project has had on student learning

  • Students who took course- majored in biology in college and knew how to do digests and electrophoresis.

  • Several of my students who chose the 6-year medical program say they couldn’t have completed, were it not for their experiences with the borrowed equipment.

  • Students show great interest and enthusiasm in DNA electrophoresis

  • Former students returning from college have told me that they are way ahead of classmates by having experienced doing these lab exercises and using this equipment.

  • I love seeing the students getting so excited!  They are working with technology that they see on TV, in the movies, and in books.  They are excited to understand what is going on in the real world!

  • Students who first experienced Biotechnology in my classroom now have their PhD’s in Molecular Biology and felt that this course opened their eyes to that field.  In addition, a recent graduate of Dartmouth College and Rhodes scholar, Megan Steven, was inspired to enter Biology after doing the DNA course.  I designed my “Topics in Advanced Biology” course in 1988 to include the molecular genetics unit.  Being able to borrow the equipment and share my “expertise” with students has been the most rewarding part of my 26 years in teaching.  It is a truly exciting experience when students have the opportunity to participate in these labs.  They remember it for years afterwards!  That “Eureka” moment when they see their first gel is so great!  I’ve also found that basic concepts of DNA fingerprinting can be taught to 4th, 5th, and 6th graders.

  • Our Biotech course ran for only 4 years, but from these students, 2 (1 Valedictorian, 1 Salutatorian) went on into Biotech – medical fields, citing our course as the reason.  Many others went on into science majors & the New Visions Medical Program as well.  It had a tremendous impact on our small school.

  • My students enjoy the labs and have gained more knowledge on DNA  

  • I once used the course with a non-regents level biology class and they loved the idea of understanding more about DNA than most of their parents could imagine.

  • AP lab students were excited about doing what we had been learning about.

  • Through the project I learned about resources (CD/VIDEO) about genetics and used them in the classroom.  We extracted lab experiences to help students learn about extracting DNA and gene splicing.

  • had one student this year who decided to go into molecular biology as a career – she didn’t even know what that was in September!

  • One student graduating this year has chosen Biotechnology as her career chose as a result of these labs.

  • My research students when working with DNA in science labs have a basic understanding of molecular biology and the techniques used to study gene reversal.

  • I have not used the information in the classroom, but I would like to start a science club that would complete some of these activities – to inspire students to further study of molecular biology.  Next year, we will teach an integrated science curriculum – so I may be able to use a lot more of the information.

  • Every year, on the news sometimes, there is a segment that shows electrophoresis (this year it was the Anthrax scare).  Many students say they saw it on television and they understand what it means! This is great!

  • One of my first students to do DNA extractions is majoring in Biology at Williams and doing summer research work there this summer.

  • The students are excited to use equipment and do lab that they’ve seen on television and the news.

  • In general, students enjoy and appreciate these labs among all others.  I feel they are important in helping students gain a concrete understand of basic biotechnological techniques that will greatly impact their lives.  Personally, I had a former student (valedictorian) return after his freshman year at Princeton to thank me for the DNA labs and explain the Southern Blot Technique to me.  (Of course he was the one who started who could squirt the micropipettes the farthest!)

  • I had a French foreign exchange student (senior) who was going to return to France to major in college as an engineer.  As a result of this lab work, he is now pursuing a career in genetics. 

  • There have been many situations where students have caught the excitement of this cutting edge technology, and therefore when given an opportunity to pursue it in scouting *, college or the community, they readily take advantage of it.

  • Students became interested and went to summer programs and became genetic counselors and engineers.

  • Gratifying to see students independent projects demonstrate a fundamental understanding of molecules and their role in genetics and disease, which would not have happened without these classes.

  • Our district uses the DNA kits during a forensic unit that we have incorporated into the Living Environment Curriculum.  The kit is instrumental in allowing the students to apply DNA technology in order to “solve a crime” each year.  In addition, our school has had increased student interest in both forensics and genetic engineering as career choices, partially due to having hands-on experience with gel electrophoresis.

  • I have several graduated working in the NYS Crime Lab.

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Appendix E

                       Responses to the question

How can we do it better? In what way(s) could the Greater Capital Region DNA Science Project help you to better implement hands-on biotechnology?

  • It would be nice if the center bulk ordered some reagents and disposable equipment so that we could buy them from the center at a reasonable cost. 

  • Providing more supplies (pipette tips and reaction tubes) could be helpful, as we do not need the quantities that you have to buy when purchasing them

  • I’m retired and I have no suggestions for change.  But, I feel strongly that bright students in “poor” or “stingy” districts NEED hands-on experience with biotechnology labs.  My budget at Hudson had been reduced to half of what it was10 years ago despite rising costs and my trips to board meetings.  Bud Bertino’s class for AP teachers is a good idea, but perhaps a program similar to the one at New Paltz, where area teachers could transport other AP and honor students to a location where biotechnology labs were provided, would help more students get a good introduction.  It could support many new teachers who are assigned AP Bio in the wake of retirement of people who took earlier courses. 

  • Providing a list of materials that need to be ordered to do the labs; we used to have these things already provided. 

  • To be honest with you, the training and equipment are more than we could ask for!  There are many schools that cannot afford the equipment – especially for all of their students in the lab setting.  For me, however, it was extremely helpful when we could order the supplies for the labs through SUNY! I cannot justify/afford the supplies through our middle school budget.  Thank you for everything! You have made a tremendous difference in teaching in the capital district!

  • Provide “refresher” or update mini-sessions for those of us who have attended the 2-week workshops. 

  • You have done a great job in making this material available to schools.

  • I enjoyed the program and would recommend it to other teachers.  Once, I had to do order my own materials and not be able to borrow equipment, I just eliminated it from the curriculum.  The students at Shenendahowa got a chance to do electrophoresis in the High School. 

  • I don’t know.  I thought it was pretty good the way it was!

  • Requiring checks before ordering is very inconvenient in our organization.  Please accept purchase orders!  #2. Include email addresses ( & phone #’s) or “on-call experts” to answer questions.  For those who are new and/or uncertain, professionals that can be reached would be helpful!  #3. For an additional fee/stipend, could we get retired teachers in to do demos or labs with kids or just help us beginners to set up!

  • Just keep offering affordable classes!

  • At this point, just to continue supplying the equipment would be the most useful.  Contacts of suggested outlets for supplies would also be useful in light of the lack of supplies with the kits. 

  • Provide mentors for new teachers in the skills and technologies of DNA manipulation.

  • I talk about the experiments when I am reviewing DNA for the Intermediate Level Science Assessment. 

  • When we used to get Dye samples, it was great!  The dyes worked out perfectly to the scenario set by the workshop.  I’ve asked for the formula of the dyes, but so far I’ve not been successful in getting it.  If you could provide us with the formulas to make them, or make them available for purchase, that would be the best news I could receive!  We purchase kits from Science Kit, but the dyes only vary by two, not 3 or 4 like the workshop dyes did.  It’s caused a lot of problems for my scenarios we design for the forensic unit.  Other than that, everything else is perfect – don’t change a thing!

  • I am at a small district and it would be helpful if there were a way to get the materials as well as the equipment once again. 

  • New mini-workshops.  Exact order form, cost and instructions, briefly.  When labs take too long to figure out what to order and how to set up, we (I) don’t do them.  We have 8 bio teachers at Albany H.S. – maybe someone could come to our school and do protein electrophoresis for us.  Chemistry teachers might come too.

  • I have stopped using the equipment the last few years simply because the prep time is phenomenal since the grant ran out for the lab assistant.  I was teaching Bio in a small school, by myself, and it was just too much for one person to undertake.  This year I began teaching a (slightly) larger school, where there are 2-3 Bio teachers and I would like to start using the equipment and labs again. 

  • Get my district to increase prep time.  Really, you did a great job.

  • I would appreciate being able to borrow the equipment for longer periods of time. (Luckily I have been able to do this in the past).  *Including a trip to the National Institute of Health.

  • Excellent program – continue. Thanks Linda A.

  • Keep providing equipment and in-service training.  A time to share experiences and to try new labs would be helpful.

  • Continue - Continue - Continue.

  • It would be nice to be able to get dyes and materials; not just equipment.

  • You’re doing a great job.  Keep making us aware of “freebie” that go with this program.

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